GST 抗体
Quick Overview for GST 抗体 (ABIN129505)
抗原
See all GST 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-200
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Supplier
- Rockland
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原理
- GST Antibody
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交叉反应 (详细)
- Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
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产品特性
- Synonyms: mouse anti-GST antibody, Glutathione-S-Transferase, Anti-GST monoclonal antibody
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纯化方法
- Anti-GST monoclonal antibody was purified from concentrated tissue culture supernate by Protein A chromatography which reacts specifically with GST.
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过滤
- Sterile filtered
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免疫原
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Immunogen: A BALB/c mouse was immunized with Glutathione-S-Transferase [Schistosoma japonicum].
Immunogen Type: Native Protein
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亚型
- IgG
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-GST antibody has been tested by ELISA and western blot and is suitable for most immunological techniques requiring high titer binding and lot-to-lot consistency.
Western Blot Dilution: 1:1,000 - 1:10,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:50,000 - 1:150,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store anti-GST at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- GST (Glutathione S Transferase (GST))
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别名
- GST
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背景
- Background: Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
抗原
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