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CDK1 抗体

Rockland 200-301-160 CDK1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal POH-1 unconjugated
Rockland
产品编号 ABIN129504
Supplier Product No.: 200-301-160
发货至: 中国
  • 抗原 See all CDK1 抗体
    CDK1 (Cyclin-Dependent Kinase 1 (CDK1))
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    小鼠
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    单克隆
    标记
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    This CDK1 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Supplier Product No.
    200-301-160
    Supplier
    Rockland
    原理
    Cdc2 p34 Antibody
    交叉反应 (详细)
    This antibody is not cross reactive with other cyclin dependent kinases.
    产品特性
    Synonyms: mouse anti-Cdc2 antibody, mouse anti-p34 antibody, Cell Division Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, p34 Cdk1 antibody, p34 protein kinase antibody, CDC28A, CDKN1, P34CDC2
    纯化方法
    This protein A purified mouse monoclonal antibody reacts specifically with p34 cdc2 in human tissues and cell lines.
    过滤
    Sterile filtered
    免疫原

    Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.

    Immunogen Type: Recombinant Protein

    克隆位点
    POH-1
    亚型
    IgG2a kappa
    Top Product
    Discover our top product CDK1 Primary Antibody
  • 应用备注

    Application Note: Anti-p34 cdc2 has been tested by western blot and is suitable for immunohistochemistry, immunoprecipitation (as active kinase), and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products.  HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting.  LEP fibroblast cell lysate is suggested as a negative control.   Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1 % saponin treatment or other permeabilization method.

    Western Blot Dilution: 1:500 - 1:1,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
    有效期
    12 months
  • Ye, Fincher, Tang, McNeal, Gygax, Wexler, Ryan, James, Osmani: "Proteolysis and tyrosine phosphorylation of p34cdc2/cyclin B. The role of MCM2 and initiation of DNA replication to allow tyrosine phosphorylation of p34cdc2." in: The Journal of biological chemistry, Vol. 272, Issue 52, pp. 33384-93, (1998) (PubMed).

    Palmer, Gavin, Nebreda: "A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1." in: The EMBO journal, Vol. 17, Issue 17, pp. 5037-47, (1998) (PubMed).

  • 抗原
    CDK1 (Cyclin-Dependent Kinase 1 (CDK1))
    别名
    CDK1 (CDK1 产品)
    背景
    Background: p34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF).  The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues.  In humans the phosphorylation appears to be performed by p60src.  The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins.  The phosphorylation activity is coupled to the entry into the M-phase of the cell.  p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally.  Association with deletion mutants of cyclin halts the M-phase before it is completed.
    基因ID
    983
    NCBI登录号
    NP_001307847
    UniProt
    P06493
    途径
    Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA
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