Western blot: 0.5-4 μg/mL. Immunohistochemistry: 10-20 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Tumor necrosis factor receptor 1 and 2 (TNF-R1 and TNF-R2) are 55 and 75 kDa members of a family cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, Ox40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. While TNF-R1 and TNF-R2 share 28 % sequence homology in the extracellular domains, their intracellular domains lack sequence homology, suggesting that they differ in their internal signal transduction pathways. TNF-R1 contains an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF-R1 associated death domain protein), and subsequent interactions with FADD. TNF-R1 can also activate the transcription factor NFκB via TRAF2 (TNF receptor associated factor 2). The cytoplasmic domain of TNF-R1 can directly interact with Jak kinase, thereby activating the JAK/STAT signal transduction cascade.Synonyms: TNF-R1, TNF-RI, TNFR-I, Tnfrsf1a, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor type I, p55, p60