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- 抗原 See all YES1 抗体
- YES1 (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This YES1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- AM00159PU-N specifically recognizes Yes at 59 kDa.
- 产品特性
- Synonyms: YES, p61-Yes, c-Yes, Proto-oncogene tyrosine-protein kinase Yes
- 纯化方法
- Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
- 组件
- incl. positive Control
- 免疫原
- Synthetic peptide conjugated to KLH
- 克隆位点
- 2B8
- 亚型
- IgG1
- Top Product
- Discover our top product YES1 Primary Antibody
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anti-V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1) (pTyr426) antibody
YES1 适用: 人, 小鼠, 大鼠 WB, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1) (AA 1-155) antibodyYES1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1) (AA 1-543) antibodyYES1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2D5 unconjugated
anti-V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1) (AA 1-80) antibodyYES1 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3C6 unconjugated
anti-V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1) (AA 441-541) antibodyYES1 适用: 人, 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1) (AA 1-543) antibodyYES1 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 2F4 unconjugated
anti-V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1) (AA 284-541) antibodyYES1 适用: 人, 小鼠 WB, IHC, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1) (Center) antibodyYES1 适用: 人, 小鼠 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1) antibodyYES1 适用: 人, 犬, 斑马鱼 WB, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1) (AA 1-250) antibodyYES1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Use A431 untreated cell lysate as Positive Control. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Lyophilized Cell Lysate from serum starved A431 cellsReconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control is recommended for immunoblot applications. 20 µl of positive controlcell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation!
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 1 mL H2O (15 min, RT).
- 缓冲液
- 1 mL2 x PBS / 0.09 % Sodium Azide / PEG and Sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
- 有效期
- 12 months
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- 抗原
- YES1 (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1))
- 别名
- YES1 (YES1 产品)
- 背景
- Yes is a member of a family of 9 tyrosine kinases regulating cellular responses to extracellular stimuli. Yes, src and fyn are ubiquitously expressed while the other members are primarily expressed in hematopoietic cells. Each protein tyrosine kinase (PTK) contains three domain (SH1, SH2, SH3). The SH1 domain has PTK activity, while the SH2 and SH3 domains are invloved in mediating protein-protein interactions by binding phosphotyrosine- containing and proline-riche motifs. By means of SH2-phosphotyrosine binding, PTKs are able to associate with activated receptor protein tyrosine kinases, e.g. platelet-derived growth factor (PDGFR)- and epidermal growth factor (EGF)- receptor.Synonyms: Proto-oncogene tyrosine-protein kinase Yes, YES, c-Yes, p61-Yes
- 基因ID
- 7525
- UniProt
- P07947
- 途径
- CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling
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