Vimentin 抗体
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- 抗原 See all Vimentin (VIM) 抗体
- Vimentin (VIM)
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适用
- 人, 小鼠, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Vimentin antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 特异性
- This antibody specifically recognizes Vimentin at 54 kDa in Western-blot applications.
- 纯化方法
- Size exclusion chromatography
- 组件
- incl. positive Control
- 免疫原
- Synthetic peptide conjugated to hemocyanin
- 克隆位点
- 11H6
- 亚型
- IgG
- Top Product
- Discover our top product VIM Primary Antibody
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- 应用备注
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 1 mL H2O (15 min, RT).
- 缓冲液
- 1 mL2 x PBS containing 0.09 % Sodium Azide/PEG and Sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C/-80 °C
- 储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- Vimentin (VIM)
- 别名
- Vimentin (VIM 产品)
- 背景
- Vimentin is a major subunit protein of intermediate filaments found in mesenchymal cells. Vimentin is expressed in many tumors/tumor cell lines after epithelial to mesenchymal transition.Synonyms: VIM
- 分子量
- 54 kDa
- 基因ID
- 7431
- UniProt
- P08670
- 途径
- Caspase Cascade in Apoptosis
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