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VASP 抗体 (pSer239)

Name: VASP 抗体 (pSer239)
SKU: ABIN126913
Availability: OutOfStock

VASP 适用: 人, 小鼠 WB, FACS, EIA 宿主: 小鼠 Monoclonal 2-20E-012 unconjugated

Western Blotting (WB) image for anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer239) antibody (ABIN126913) (VASP 抗体 (pSer239))

1 image

产品编号 ABIN126913

发货至: 中国

Datasheet as PDF

抗原 See all VASP 抗体

VASP (Vasodilator-Stimulated phosphoprotein (VASP))
抗原表位
16

16

15

8

5

5

4

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer239
适用
123

57

53

11

8

8

5

2

2

2

1

1
人, 小鼠
宿主
106

16

2
小鼠
Compatible Secondaries
克隆类型
102

22
单克隆
标记
79

7

4

3

3

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This VASP antibody is un-conjugated
应用范围
97

48

45

28

22

14

14

14

12

11

4

4

4

1
Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

特异性

This antibody recognizes VASP only, when Ser239 is phosphorylated, a site preferred by cGMP-dependent protein kinase (PKG) but also used by cAMP-dependent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, antibody VASP-22E11 is able to monitor the phosphorylation state of VASP serine 239 as well as PKA activity.

产品特性

Synonyms: Vasodilator-stimulated phosphoprotein

纯化方法

Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

组件

incl. positive Control

免疫原

Synthetic phosphopeptide conjugated to KLH.

克隆位点

2-20E-012

亚型

IgG1

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anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibody
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anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibody
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anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer239) antibody
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anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-380) antibody
VASP 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer239) antibody
VASP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer238) antibody
VASP 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-380) antibody
VASP 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 78-114), (N-Term) antibody
VASP 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: Use at 0.05 μg/mL. Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Flow Cytometry. Included Positive Control: Cell lysate from Forskolin-treated HepG2 cells (See Protocols formore details).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

实验流程

Positive Control Cell Lysate: HepG2 Forskolin treatedFormat: Lyophilized cell lysate from HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.

限制

仅限研究用
溶解方式

Restore with 1 mL H2O (15 min, RT).

缓冲液

2 x PBS/0.09 % Na-azide/PEG and Sucrose.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C/-80 °C

储存方法

Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

有效期

12 months
抗原

VASP (Vasodilator-Stimulated phosphoprotein (VASP))

别名

VASP (VASP 产品)

背景

VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines, particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation.Synonyms: Vasodilator-stimulated phosphoprotein

基因ID

7408

UniProt

P50552

途径

TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation