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Phosphotyrosine 抗体 (phosphorylated)

Name: Phosphotyrosine 抗体 (phosphorylated)
SKU: ABIN126876
Availability: OutOfStock

WB, IP, EIA 宿主: 小鼠 Monoclonal 9F1 unconjugated

Western Blotting (WB) image for anti-Phosphotyrosine (phosphorylated) antibody (ABIN126876) (Phosphotyrosine 抗体 (phosphorylated))

1 image

产品编号 ABIN126876

发货至: 中国

Datasheet as PDF

抗原 See all Phosphotyrosine products

Phosphotyrosine
抗原表位
22

2

1

1

1

1

1

1

1
phosphorylated

适用

请咨询
宿主
84

27

1
小鼠
Compatible Secondaries
克隆类型
94

18
单克隆
标记
35

17

10

8

4

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Phosphotyrosine antibody is un-conjugated
应用范围
79

59

38

38

32

22

21

17

13

9

7

7

7

6

3

2

2

2

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

纯化方法

Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

组件

incl. positive Control

免疫原

Synthetic phosphopeptide conjugated to KLHEpitope: . . . pYpY. . .

克隆位点

9F1

亚型

IgG1
anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Phosphotyrosine antibody
适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated

anti-Phosphotyrosine antibody
WB, ELISA 宿主: 小鼠 Monoclonal unconjugated

anti-Phosphotyrosine antibody
适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 3F6 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, FACS, ISt, StM 宿主: 小鼠 Monoclonal PY20 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 unconjugated

anti-Phosphotyrosine antibody (HRP)
WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 HRP

anti-Phosphotyrosine antibody (FITC)
WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 FITC

anti-Phosphotyrosine antibody (PerCP)
WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 PerCP

anti-Phosphotyrosine antibody (Atto 594)
WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Atto 594

应用备注

ELISA: Use at 0.05 μg/mL. Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Immunoprecipitation: Use at 1-10 μg per 10^6 pervanadate-treated A431 cells. Included Positive Control: Phosphotyrosine MW standard.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
溶解方式

Restore with 1 mL H2O (15 min, RT).

缓冲液

1 mL2 x PBS with 0.09 % Sodium Azide/PEG and Sucrose.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C/-80 °C

储存方法

Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

有效期

12 months
抗原

Phosphotyrosine

Abstract

Phosphotyrosine 产品

物质类

Amino Acid

背景

Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.