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- 抗原 See all Phosphothreonine products
- Phosphothreonine
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抗原表位
- phosphorylated
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Phosphothreonine antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- 交叉反应 （详细）
- Species reactivity (tested):Human, Mouse, Rat and Dog.
- 纯化方法
- Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
- 组件
- incl. positive Control
- 免疫原
- Synthetic phosphopeptide conjugated to KLH
- 克隆位点
- 14B3
- 亚型
- IgG1
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anti-Phosphothreonine antibody
WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphothreonine antibody (FITC)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal FITC
anti-Phosphothreonine antibody (Biotin)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-Phosphothreonine antibody (Atto 594)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Atto 594
anti-Phosphothreonine antibody (Atto 488)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-Phosphothreonine antibody (Atto 390)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Atto 390
anti-Phosphothreonine (phosphorylated) antibody (HRP)WB, IP, ELISA, IHC 宿主: 兔 Polyclonal HRP
anti-Phosphothreonine (pThr) antibodyWB, IP, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphothreonine antibodyIP, ELISA, IHC, FACS, RIA 宿主: 小鼠 Monoclonal 18F6 unconjugated
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- 应用备注
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ELISA: 0.05 μg/mL. Immunoprecipitation: 1-10 μg per 10^6 pervanadate-treated A431 cells. Western Blot (Immunoblotting): 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking buffer. DO NOT USE MILKOR CASEIN FOR BLOCKING!
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Positive Control: pSer / pThr Molecular Weight MarkerFormulation:
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 1 mL H2O (15 min, RT).
- 缓冲液
- 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C/-80 °C
- 储存方法
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Store lyophilized (preferably in a desiccator) at -20 °Cand reconstituted (aliquote and freeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 1 week.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- Phosphothreonine
- Abstract
- Phosphothreonine 产品
- 物质类
- Amino Acid
- 背景
- Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases.
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