-
- 抗原 See all Phosphoserine products
- Phosphoserine
-
抗原表位
- phosphorylated
- 适用
- 请咨询
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Phosphoserine antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- 纯化方法
- Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
- 组件
- incl. positive Control
- 免疫原
- Synthetic phosphopeptide conjugated to KLH.
- 克隆位点
- 4A3
- 亚型
- IgM
-
anti-Phosphoserine antibody
WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphoserine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 7A3 unconjugated
anti-Phosphoserine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphoserine antibodyWB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphoserine antibody (FITC)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal FITC
anti-Phosphoserine antibody (Biotin)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-Phosphoserine antibody (Atto 594)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 594
anti-Phosphoserine antibody (Atto 488)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-Phosphoserine antibody (Atto 390)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 390
anti-Phosphoserine antibodyWB, ELISA, IP, IHC, RIA, FACS 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
ELISA: Use at 0.05 μg/mL. Immunoprecipitation: Use at 1-10 μg per 10^6 pervanadate-treated A431 cells. Western Blot (Immunoblotting): 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking buffer. DO NOT USE MILKOR CASEIN FOR BLOCKING!
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Positive Control: pSer / pThr Molecular Weight MarkerFormulationThe pSer/pThr molecular weight marker contains rabbit muscle phosphoproteins isolatedby Fe+/IDA-affinity chromatography. Proteins are lyophilized fromPBS/NaF/PEG/SUCROSE/Bromophenolblue and Na-azide. After reconstitution the solutioncontains 0. 09% Na-azide. StabilityReconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C. ApplicationThe pSer/pThr molecular weight marker is recommendede for immunoblot applications. Use 20 µl molecular meight marker per lane. Note: Use BSA based blot incubation buffers. Milk, Casein and Blotto might interfere withantibody - antigeninteraction. StorageAliquote and store frozen. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
- 限制
- 仅限研究用
-
- 溶解方式
- Restore with 1.0 mL H2O (15 min, RT).
- 缓冲液
- 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C/-80 °C
- 储存方法
-
Store lyophilized (preferably in a desiccator) at -20 °Cand reconstituted (aliquote and freeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 1 week.
Shelf life: one year from despatch. - 有效期
- 12 months
-
- 抗原
- Phosphoserine
- Abstract
- Phosphoserine 产品
- 物质类
- Amino Acid
- 背景
- Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases.
-
You are here:
