Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
特异性
The monoclonal antibody decorates the karyoskeleton, i.e. the intermediate filament equivalent of the nucleus. Lamin isotypes of Mr 60-75 kDa. Xenopus laevis LII, LIII Bovine LB1, B2 Mouse LB2 Rat + Human LB1, B2 Trout LII, LIII Rat kangaroo LII, LIII
纯化方法
Protein A affinity chromatography.
免疫原
Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells).
Immunofluorescence microscopy. Immunoblotting. Immunohistochemistry: 1/10 for 1 h at RT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in 1 mL distilled water.
注意事项
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.