Flow Cytometry: Membrane permeabilisation is required for this application. ELISA: 5 μg/mL - 10 μg/mL. Functional Assays. Immunohistochemistry on frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
This 17 kDa cytokine is expressed by activated macrophages, monocytes, neutrophils, T cells and NK cells.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2