M13 Bacteriophage G8P Capsid 抗体
适用: Bacteriophage M13
WB, EIA, IEM
宿主: 小鼠
Monoclonal
B62-FE2
unconjugated
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- 抗原
- M13 Bacteriophage G8P Capsid
- 适用
- Bacteriophage M13
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoelectron Microscopy (IEM)
- 特异性
- B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT (See Ref. 2).
- 纯化方法
- Affinity Chromatography on Protein A
- 免疫原
- fd phages from E. coli F+ strain (JM109).
- 克隆位点
- B62-FE2
- 亚型
- IgG2b
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anti-M13 Bacteriophage G8P Capsid antibody
适用: Bacteriophage M13 WB, FACS, ICC, ELISA, IHC 宿主: 小鼠 Monoclonal LRL-ph2 unconjugated
anti-M13 Bacteriophage G8P Capsid antibody适用: Bacteriophage M13 WB, FACS, ICC, ELISA 宿主: 小鼠 Monoclonal RL-ph1 unconjugated
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- 应用备注
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Phage display: immunoassay for identification of recombinant antigen- orantibody-phages, Detection limit: 107 phage particles. Immunoblotting. Immunoelectron microscopy. ELISA: 1: 50 000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in 0.1 mL dist. water.
- 浓度
- 5.0 mg/mL
- 缓冲液
- 10 mM Tris-HCl buffer, pH 8.0 containing 300 mM NaCl
- 注意事项
- This product is photosensitive and should be protected from light
- 储存条件
- 4 °C
- 储存方法
- Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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- 抗原
- M13 Bacteriophage G8P Capsid
- 物质类
- Phage Protein
- 背景
- M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.Synonyms: Coat protein B, Gene 8 protein, Major coat protein
- 基因ID
- 927333
- NCBI登录号
- NP_510890
- UniProt
- P69541
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