PRDX6-PS2 抗体 (C-Term)
PRDX6-PS2
适用: Arabidopsis thaliana
ELISA, WB
宿主: 兔
Polyclonal
unconjugated
(2 references)-
- 抗原
- PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))
- 抗原表位
- C-Term
- 适用
- Arabidopsis thaliana
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- ELISA, Western Blotting (WB)
- 交叉反应 （详细）
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 25 / 20 kDa
- 纯化方法
- serum
- 免疫原
- thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of AtGPX7 accession At4g31870 has been used for antibody production
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- 应用备注
- 1: 5000 with alkaline phosphatase system (AP) (WB), 1:1, 1:2, 1:4, 1:8, 1: 16 (ELISA)
- 说明
-
This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 100 μL of sterile water.
- 注意事项
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
- 储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
-
: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." in: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, (2013) (PubMed).
: "Decarbonylated cyclophilin A Cpr1 protein protects Saccharomyces cerevisiae KNU5377Y when exposed to stress induced by menadione." in: Cell stress & chaperones, (2010) (PubMed).
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: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." in: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, (2013) (PubMed).
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- 抗原
- PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))
- 别名
- GPX
- 背景
-
AGI Code: At4g31870
GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX. - 分子量
- expected: 25 kDa, apparent: 20 kDa
- UniProt
- Q9SZ54
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