GST-Tag 抗体
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- 抗原 See all GST-Tag products
- GST-Tag
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This GST-Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 特异性
- The antibody S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibod is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.
- 交叉反应 (详细)
- Recognizes fusion proteins in all species
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- GST fusion protein
- 克隆位点
- S-tag-05
- 亚型
- IgG2b
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- 应用备注
- Western blotting: Recommended dilution: 1 μg/mL, positive control: GST fusion protein, reducing conditions.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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- 抗原
- GST-Tag
- 别名
- GST tag (GST-Tag 产品)
- 物质类
- Tag
- 背景
- The GST (glutathione-S-transferase) is a 26 kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors.
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