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- 抗原 See all ITGAL 抗体
- ITGAL (Integrin alpha-L (ITGAL))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ITGAL antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)
- 特异性
- The antibody MEM-83 reacts with an extracellular epitope of CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
- 交叉反应 (详细)
- Human
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Human peripheral blood lymphocytes
- 克隆位点
- MEM-83
- 亚型
- IgG1
- Top Product
- Discover our top product ITGAL Primary Antibody
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- 应用备注
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Functional application: The antibody MEM-83 directly induces the binding of T cells to purified ICAM-1. Using an in vitro-translated CDlla cDNA deletion series, the MEM-83 activation epitope was mapped to the "I" domain of the LFA-1 alpha subunit. The studies have therefore identified a novel LFA-1 activation epitope mapping to the I domain of LFA-1, which could play a role in the regulation of LFA-1 binding to ICAM-1.
Flow cytometry: Recommended dilution: 1 μg/mL. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4
- 储存液
- Azide free
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3." in: The Journal of cell biology, Vol. 126, Issue 2, pp. 529-37, (1994) (PubMed).
: "A novel LFA-1 activation epitope maps to the I domain." in: The Journal of cell biology, Vol. 120, Issue 6, pp. 1519-27, (1993) (PubMed).
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Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3." in: The Journal of cell biology, Vol. 126, Issue 2, pp. 529-37, (1994) (PubMed).
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- 抗原
- ITGAL (Integrin alpha-L (ITGAL))
- 别名
- CD11a (ITGAL 产品)
- 别名
- CD11A antibody, LFA-1 antibody, LFA1A antibody, (p180) antibody, Cd11a antibody, LFA-1A antibody, Ly-15 antibody, Ly-21 antibody, CD11a antibody, ITGAL antibody, integrin subunit alpha L antibody, integrin alpha L antibody, ITGAL antibody, Itgal antibody
- 背景
- Integrin subunit alpha L,CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.,LFA-1, LFA1A, ITGAL
- 基因ID
- 3683
- UniProt
- P20701
- 途径
- Activated T Cell Proliferation, Integrin Complex
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