The polyclonal antibody DR-264 binds with high affinity to vitellogenin from zebrafish (Danio rerio). The antibody also cross-reacts with vitellogenin from other cyprinid species, including common carp (Cyprinus carpio), fathead minnow (Pimephales promelas) and roach (Rutilus rutilus).The degree of cross-reactivity differs between species and with the methods employed.
纯化方法
Protein-G affinity-purified
免疫原
Vitellogenin purified from plasma of 17ß-estradiol treated zebrafish
DR-264 is well suited for ELISA and immunoblotting for several cyprinid species. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating. Normal dilution range: ELISA: 1:100 - 1: 2000, Western blot: 1:100 - 1:50
限制
仅限研究用
状态
Liquid
缓冲液
The antibodies are dissolved in 0.1M Na-phosphate, 0.15M NaCl, 1% BSA, 0.1% Na-azide, pH 7.4.