Immunocytochemistry. Immunohistochemistry on formalin-fixed paraffin embedded tissues: 5-12 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.7, containing 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-80 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
TM7SF1 is an Orphan-U GPCR with an unknown ligand. This receptor has been shown to be upregulated during kidney development. TM7SF1 has been reported to be expressed in adult kidney and heart, but not in fetal kidney. ESTs have been isolated from a wide variety of tissues, including B-cell/lung/testis, brain, connective tissue, ear, embryo, eye, gall bladder, kidney, liver/spleen, lung, melanocyte/uterus/fetal heart, nerve, pineal, placenta, skin, spleen, testis, tonsil, and vessel libraries.Synonyms: Integral membrane protein GPR137B, Transmembrane 7 superfamily member 1 protein