Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
Reacts with Human Gastrin-Releasing Peptide Receptor (GRPR, Family: GPCR, Subfamily: Bombesin), 2nd extracellular domain. GRP-R has been reported in brain, breast, colon, lung, lymph node, ovary, placenta, prostate, stomach, and uterus. It has also been shown to be expressed in several cancers, including those in breast, colon, lung, ovary, pancreas, and prostate.
Western blot (5 μg/mL). Immunocytochemistry (Use acetone as fixative). Immunohistochemistry on Paraffin Embedded Sections (4 μg/mL): Steam slides in 0.01 Msodium citrate buffer, pH 6.0 at 99-100 °C for 20 min, Remove from heat and let stand atroom temperature in buffer for 20 min. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or at -20 °C to -70 °C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
GRPR mediates signals of cellular proliferation and it has been shown to be aberrantly expressed in several cancers such as colon, lung, ovary, pancreas and prostate. It also expressed at very high levels in normal pancreas and at lower levels in brain, breast, colon, lung, lymph node, ovary, placenta, prostate, stomach and uterus.Synonyms: GRP-R, GRP-preferring bombesin receptor, GRPR