Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
特异性
Reacts with G protein-coupled receptor MRGX1 (SNSR3/SNSR4) at N-term.BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MRGPRX3 (71 %).
Western Blot. Immunocytochemistry. Immunohistochemistry with formalin fixed paraffin embedded tissues: 12 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS containing 0.09 % Sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-80 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
有效期
12 months
抗原
MRGPRC
(MAS-Related G-Protein Coupled Receptor, Member C (MRGPRC))
MrgC antibody, Snsr antibody, MAS-related G-protein coupled receptor, member C antibody, Mrgprc antibody
背景
MRGX1 belongs to the Opioid Receptor subfamily because this receptor is activated by products of proenkephalin A, an opioid peptide precursor. MRGX1 has been reported exclusively in dorsal root ganglion. ESTs for MRGX1 have been identified in a human brain (hypothalamus) library and in a human cancer library.Synonyms: Mas-related G-protein coupled receptor member X1, Mrgc, Mrgprc11, Mrgprx1, Sensory neuron-specific G-protein coupled receptor 1, Snsr, Snsr1