CYSLTR1
适用: 人
WB, ELISA, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
Immunohistochemistry with formalin fixed paraffin embedded tissues: 6 -14g/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove fromheat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween(TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1 mg/mL
缓冲液
PBS, containing 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-80 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Cysteinyl leukotriene receptor 1, a Chemoattractant Receptor, is detected in spleen, peripheral blood leukocytes, and lung smooth muscle (Lynch et al., 1999). It binds cysteinyl leukotrienes, which are neutrophil chemoattractants with potent proinflammatory action. Leukotrienes mediate many features of bronchial asthma such as edema, bronchial constriction, and hyperreactivity (Bisgaard et al., 2000). Selective CysLT1 antagonists are used to treat asthma. The cysteinyl leukotrienes, leukotriene C4 (LTC4), leukotriene D4 (LTD4), and leukotriene E4 (LTE4) activate at least two receptors, CysLT1 and CysLT2, to mediate human bronchial asthma. LTC(4), LTD(4), and LTE(4) are a class of peptide-conjugated lipids formed from arachidonic acid and that are released during activation of mast cells.Synonyms: CysLTR1, Cysteinyl leukotriene D4 receptor, Cysteinyl leukotriene receptor 1, G-protein coupled receptor HG55, HG55, HMTMF81, LTD4 receptor