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GFP 抗体

Name: GFP 抗体
SKU: ABIN121945
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, IF, IP 宿主: 兔 Polyclonal unconjugated

(A) Confocal microscopy images of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP (GFP 抗体)

Immunoprecipitation of GFP-NLS from HEK293 cells using anti-GFP antibody. (GFP 抗体)

Confocal microscopy images of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP, top) or nuclear Polycomb 2-EYFP fusion protein (Pc2-EYFP, bottom). (GFP 抗体)

3 images

(4 references)

产品编号 ABIN121945

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
174

15

11

9
Aequorea victoria
宿主
64

62

34

13

9

5

2

1
兔
Compatible Secondaries
克隆类型
113

72
多克隆
标记
81

12

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
147

80

46

33

27

25

23

21

14

13

13

12

12

5

4

4

4

2

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

特异性

The antibody reacts specifically with GFP, EGFP, EYFP fusion proteins in all species.

纯化方法

Affinity Chromatography on Protein A

免疫原

EGFP, a native full-length protein

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anti-Green Fluorescent Protein (GFP) antibody
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anti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP 适用: Aequorea victoria WB, ELISA 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 兔 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)
GFP 适用: Aequorea victoria FACS, IM 宿主: 山羊 Polyclonal FITC

anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)
GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550

应用备注

Immunoprecipitation (10-20 μg/sample). Immunocytochemistry (1-3 μg/mL). Western Blotting (0.5-1.5 μg/mL). Positive control: transfected cells. Negative control: non-transfected cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS, 15 mM Sodium Azide, 0.2 % (w/v) high-grade BSA (protease free)

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

有效期

12 months
Prescher, Baumgärtel, Ivanchenko, Torrano, Bräuchle, Müller, Lamb: "Super-resolution imaging of ESCRT-proteins at HIV-1 assembly sites." in: PLoS pathogens, Vol. 11, Issue 2, pp. e1004677, (2016) (PubMed).

Gentzel, Schille, Rauschenberger, Schambony: "Distinct functionality of dishevelled isoforms on Ca2+/calmodulin-dependent protein kinase 2 (CamKII) in Xenopus gastrulation." in: Molecular biology of the cell, Vol. 26, Issue 5, pp. 966-77, (2015) (PubMed).

Ling, Wong, Li, Chan, Boukamp, Liu: "CCHCR1 interacts with EDC4, suggesting its localization in P-bodies." in: Experimental cell research, Vol. 327, Issue 1, pp. 12-23, (2014) (PubMed).

Savard, Barbaz, Bélanger, Müller-Esterl, Bkaily, Dorléans-Juste, Coté, Bovenzi, Gobeil: "Expression of endogenous nuclear bradykinin B2 receptors mediating signaling in immediate early gene activation." in: Journal of cellular physiology, Vol. 216, Issue 1, pp. 234-44, (2008) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

背景

Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (509 nm) when excited (excitation by Blue or UV light, absorption peak at 395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chromophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.Synonyms: GFP-Tag, Green fluorescent protein

UniProt

P42212