This antibody is specific for the soluble form of human CD154, also designated CD40L (sCD40 Ligand), a member of the TNF receptor superfamily, comprising the full-length of the TNF homologous region of CD154.
ELISA (direct ELISA, 0.5 μg/mL). Western blot (0.1 - 0.2 μg). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 0.1 mL distilled water.
浓度
1.0 mg/mL
缓冲液
Phosphate buffered saline pH 7.4, containing no preservatives
储存液
Without preservative
储存条件
4 °C/-20 °C
储存方法
Store lyophilized at 2 - 8 °C and reconstituted (undiluted) at -20 °C. Avoid repeated freezing and thawing. For long term storage the addition of 0.09 % sodium azide is recommended. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: One year from despatch.Reconstitution: Restore with 0.1 ml distilled water..
CD154 is expressed predominantly on activated CD4+ T lymphocytes, and acts as a costimulatory molecule in B cell differentiation/proliferation, immunoglobulin switching and germinal centre formation, through interaction with the CD40 receptor on B cells, and cytokines secreted by activated T cells.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5