Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
This antibody recognises an epitope within the C-terminal region (CT) of Caspase-1, otherwise known as IL-1Beta converting enzyme. This antibody detects a cleaved subunit band of approximately 21 kDa in heart cell lysates (predicted precursor MWT 45.2 kDa).
纯化方法
Affinity chromatography
免疫原
A 16 amino acid peptide located near human Caspase-1 carboxy terminus
Western Blot: 0.5 μg - 1.0 μg/mL. Immunohistochemistry on paraffin sections: 0.5 μg - 1.0 μg/mL, This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Recommended Positive Control: Human heart tissue. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS containing 0.02 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Caspase-1 is an intracellular cysteine protease, identified as a mammalian homologue to C. elegans cell death gene (ced-3). Caspase-1 has been classified as an inflammatory, rather than apoptotic caspase, due to its essential role in the cleavage of the inactive precursors of the cytokines IL-1beta and IL-18, into their mature activated and secretable forms. This regulation of pro-inflammatory cytokines by Caspase-1 has made inhibitors of Caspase-1 a possible target for use as therapeutic drugs for the treatment of inflammatory diseases.Synonyms: CASP-1, CASP1, ICE, IL1BC, IL1BCE, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45