LAT2 抗体 (AA 91-244)
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- 抗原 See all LAT2 抗体
- LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))
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抗原表位
- AA 91-244
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This LAT2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF)
- 特异性
- The antibody reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.
- 纯化方法
- Protein A affinity chromatography
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Recombinant cytoplasmic domain (aa 91-244) of human NTAL
- 克隆位点
- NAP-07
- 亚型
- IgG1
- Top Product
- Discover our top product LAT2 Primary Antibody
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- 应用备注
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Immunoprecipitation. Western Blot: Recommended dilution: 2 mg/mL, incubation 60 min Positive control: RAMOS human lymphoma cell line Negative control: HeLa human cervix carcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12 % separating SDS-PAGE gel. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. - 有效期
- 12 months
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Molecular characterization of rat gastric mucosal response to potent acid inhibition." in: Physiological genomics, Vol. 22, Issue 1, pp. 24-32, (2007) (PubMed).
: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." in: Blood, Vol. 104, Issue 1, pp. 207-14, (2004) (PubMed).
: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, (2004) (PubMed).
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Molecular characterization of rat gastric mucosal response to potent acid inhibition." in: Physiological genomics, Vol. 22, Issue 1, pp. 24-32, (2007) (PubMed).
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- 抗原
- LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))
- 别名
- LAT2 / WBSCR15 (LAT2 产品)
- 别名
- LAB antibody, NTAL antibody, WBSCR15 antibody, WBSCR5 antibody, WSCR5 antibody, LAT2 antibody, MGC139435 antibody, Ntal antibody, Wbscr5 antibody, AW125574 antibody, Wbscr15 antibody, linker for activation of T-cells family member 2 antibody, linker for activation of T cells family, member 2 antibody, LAT2 antibody, Lat2 antibody
- 背景
- NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein
- 基因ID
- 7462
- UniProt
- Q9GZY6
- 途径
- Fc-epsilon Receptor Signaling Pathway, BCR Signaling
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