MHC Class I RT1.Aa alpha-Chain (RT1.AA) 抗体
RT1.AA
适用: 大鼠
FACS, IHC (fro), IP
宿主: 小鼠
Monoclonal
OX-18
unconjugated
(1 reference)-
- 抗原 See all MHC Class I RT1.Aa alpha-Chain (RT1.AA) 抗体
- MHC Class I RT1.Aa alpha-Chain (RT1.AA)
- 适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Rat spleen glycoproteins
- 克隆位点
- OX-18
- 亚型
- IgG1
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anti-MHC Class I RT1.Aa alpha-Chain (RT1.AA) antibody
RT1.AA 适用: 大鼠 FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal OX-18 unconjugated
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- 应用备注
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Flow cytometry: use 10 μL of 1/50 - 1/100 diluted antibody to label 10^6 cells in 100 μL. (This product is routinely tested in flow cytometry on rat splenocytes). Immunohistochemistry on frozen sections: Acetone fixation is recommended - the antigen is sensitive to fixation with paraformaldehyde. Not suitable for immunohistochemistry on paraffin sections. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: MRC OX-18 has been used in immunoaffinity purification of rat MHC class I molecules. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4, containing 0.09 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. - 有效期
- 12 months
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: "[Aortic root replacement and pulmonary vein isolation, report of a case]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 2, pp. 153-6, (2008) (PubMed).
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: "[Aortic root replacement and pulmonary vein isolation, report of a case]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 2, pp. 153-6, (2008) (PubMed).
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- 抗原
- MHC Class I RT1.Aa alpha-Chain (RT1.AA)
- 别名
- MHC Class I (RT1Aa) (RT1.AA 产品)
- 别名
- RTA antibody, Rt1.aa antibody, RT1 class I, locus A antibody, RT1-A antibody
- 背景
- MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: RT1 class I histocompatibility antigen AA alpha chain
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