Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)
特异性
The antibody reacts with Linker for activation of T cells (LAT), a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. Does not react with mouse.
产品特性
Synonyms: pp36, p36-38, 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-CellsFamily Member 1
纯化方法
Protein A affinity chromatography
纯度
> 95 % (by SDS-PAGE)
免疫原
Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmicdomain of human LAT
Immunoprecipitation. Western Blot (Tyrosine-phosphorylated form of LAT is not optimally recognized). Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
LAT antibody, lat antibody, LAT1 antibody, pp36 antibody, linker for activation of T-cells antibody, linker for activation of T cells antibody, LAT antibody, lat antibody, Lat antibody
背景
LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FceRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.Synonyms: 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-Cells Family Member 1, p36-38, pp36