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- 抗原 See all Blood Group Lewis B products
- Blood Group Lewis B
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Blood Group Lewis B antibody is un-conjugated
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Enzyme Immunoassay (EIA)
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 纯化方法
- Affinity Chromatography on Protein A.
- 免疫原
- Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification)
- 克隆位点
- 2-25LE
- 亚型
- IgG1
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anti-Blood Group Lewis B antibody
适用: 人 IHC (p), WB, ELISA 宿主: 小鼠 Monoclonal 2-25LE unconjugated
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- 应用备注
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Immunohistochemistry: 2-4 μg/mL for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Use human colon CA for postive control. Western blot. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4, with 15 mM Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store at 2-8 °C for 6 months. For long-term storage aliquot and store at -20 °C. Avoid freeze/thaw cycles.
Shelf Life: one year from despatch. - 有效期
- 12 months
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- 抗原
- Blood Group Lewis B
- Abstract
- Blood Group Lewis B 产品
- 别名
- MUC5 antibody, TBM antibody, leB antibody, mucin 5AC, oligomeric mucus/gel-forming antibody, MUC5AC antibody
- 背景
- Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.Synonyms: Blood Group Antigen Lewis b
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