Transferrin Receptor 抗体
-
- 抗原 See all Transferrin Receptor (TFRC) 抗体
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Transferrin Receptor antibody is un-conjugated
-
应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
- 交叉反应 (详细)
- Species reactivity (tested):Human.
- 纯化方法
- Affinity Chromatography on Protein A
- 免疫原
- KG1 human acute myelogenous leukaemia cell line
- 克隆位点
- MEM-189
- 亚型
- IgG1
- Top Product
- Discover our top product TFRC Primary Antibody
-
-
- 应用备注
-
Flow cytometry: use 5 μL of neat antibody to label 10^6 cells Immunoprecipitation. Western Blotting (non reducing conditions).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- TBS buffer pH 8.0, 15 mM Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
-
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. - 有效期
- 12 months
-
- 抗原
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
- 别名
- CD71 / TFRC (TFRC 产品)
- 背景
- CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH . CD71 is also involved in uptake of non-transferrin bound iron.Synonyms: TfR1, Transferrin receptor protein 1, p90
- UniProt
- P02786
- 途径
- Transition Metal Ion Homeostasis
-