CD3D 抗体
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- 抗原 See all CD3D 抗体
- CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD3D antibody is un-conjugated
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应用范围
- Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody recognizes Rat CD3, a 25 kDa antigen which is found on rat T-cells. This antibody does not react with Rat B cells. In immunohistology it stains Rat thymus tissues strongly in the medulla and weakly in the cortex.
- 交叉反应 （详细）
- Species reactivity (tested):Rat.
- 纯化方法
- Ammonium Sulphate Precipitation.
- 免疫原
- F344 rat T cells stimulated with PMA (TPA) and calcium ionophore. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag8.653 mouse myeloma cell line.
- 克隆位点
- 1F4
- 亚型
- IgM
- Top Product
- Discover our top product CD3D Primary Antibody
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anti-CD3d Molecule, delta (CD3-TCR Complex) (CD3D) (AA 127-171) antibody
CD3D 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 4B9G9 unconjugated
anti-CD3d Molecule, delta (CD3-TCR Complex) (CD3D) (AA 127-171) antibodyCD3D 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 6B6B12 unconjugated
anti-CD3d Molecule, delta (CD3-TCR Complex) (CD3D) (AA 22-105) antibodyCD3D 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1F6A8 unconjugated
anti-CD3d Molecule, delta (CD3-TCR Complex) (CD3D) (AA 18-113) antibodyCD3D 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD3d Molecule, delta (CD3-TCR Complex) (CD3D) (AA 1-171) antibodyCD3D 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-CD3d Molecule, delta (CD3-TCR Complex) (CD3D) (AA 22-105) antibodyCD3D 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD3d Molecule, delta (CD3-TCR Complex) (CD3D) (AA 127-171) antibodyCD3D 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1H12G5 unconjugated
anti-CD3d Molecule, delta (CD3-TCR Complex) (CD3D) antibodyCD3D 适用: 人, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD3d Molecule, delta (CD3-TCR Complex) (CD3D) (Internal Region) antibodyCD3D 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD3d Molecule, delta (CD3-TCR Complex) (CD3D) (AA 22-106) antibodyCD3D 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Flow cytometry: 1/10 - 1/25, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections: 1/10 - 1/25. Immunohistochemistry on paraffin sections: 1/10.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS containing 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))
- 别名
- CD3 delta Chain (CD3D 产品)
- 别名
- CD3-DELTA antibody, T3D antibody, CD3 antibody, T3d antibody, CD3d molecule antibody, CD3 antigen, delta polypeptide antibody, CD3D antibody, Cd3d antibody
- 背景
- T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: CD3D, T-cell surface glycoprotein CD3 delta chain, T3D
- 基因ID
- 25710
- NCBI登录号
- NP_037301
- UniProt
- P19377
- 途径
- TCR Signaling, CXCR4-mediated Signaling Events
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