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CD3D 抗体 (FITC)

This anti-CD3D antibody is a 小鼠 单克隆 antibody detecting CD3D in FACS. Suitable for 大鼠.
产品编号 ABIN120369
发货至: 中国

Quick Overview for CD3D 抗体 (FITC) (ABIN120369)

抗原

See all CD3D 抗体
CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

适用

  • 59
  • 31
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  • 2
大鼠

宿主

  • 87
  • 28
  • 2
  • 2
  • 1
  • 1
小鼠

克隆类型

  • 83
  • 38
单克隆

标记

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  • 23
  • 15
  • 4
  • 2
  • 2
  • 2
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  • 2
This CD3D antibody is conjugated to FITC

应用范围

  • 87
  • 59
  • 44
  • 28
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  • 3
  • 1
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Flow Cytometry (FACS)

克隆位点

1F4
  • 特异性

    SM253F recognises rat CD3, a 25 kDa antigen which is found on rat T cells. SM253F does not react with rat B cells.

    免疫原

    F344 rat T cells stimulated with PMA (TPA) and calcium ionophore.

    亚型

    IgM
  • 应用备注

    Flow cytometry: Use 10 μL of Neat antibody to label 10^6 cells in 100 μL. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T- cells in the presence of PMA (2). We recommend antibody ABIN120367 for this purpose.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.1 mg/mL

    缓冲液

    PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in alqiuots) at -20 °C for longer.
  • 抗原

    CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

    别名

    CD3 delta Chain

    背景

    T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: CD3D, T-cell surface glycoprotein CD3 delta chain, T3D

    基因ID

    25710

    NCBI登录号

    NP_037301

    UniProt

    P19377

    途径

    TCR Signaling, CXCR4-mediated Signaling Events
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