This antibody recognises CD8 alpha. Double-staining with clone Du CD8-1 and duck IgY light chain clone 14A3 (product code SM2274P), revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+/14A3+ cells in duck bursa. (1)
纯化方法
Affinity Chromatography on Protein G.
免疫原
293T cells expressing Pekin Duck CD8 alpha. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
CD8B
适用: Cow, 山羊, 绵羊, Water Buffalo
FACS
宿主: 小鼠
Monoclonal
CC58
PE
应用备注
Flow Cytometry. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS containing 0.09 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD8 alpha shown to be expressed by thymocytes, splenocytes, peripheral lymphoid cells and the vast majority of bursal cells. Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs: the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA).Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain