Flow Cytometry: 1/25 - 1/50, use 10 μL of the suggested working dilution to label 106 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4 containing 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
TNFSF18
(Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))
GITRL is a 20 kD member of the tumour necrosis factor ligand superfamily, otherwise known as TNSF18. Mouse GITRL is predominantly expressed on immature and mature dendritic cells, macrophages and B cells. Expression of mouse GITRL can be transiently up regulated on these cells, following lipolysaccharide (LPS) stimulation, but protein expression is rapidly down modulated shortly after stimulation. Studies demonstrate that GITRL can neutralise the suppressive effects of CD4+CD25+ regulatory T cells and can also provide a co-stimulatory signal for both naive and primed T cells, suggesting a key role for GITRL in the regulation of T cell-mediated immunity.Synonyms: AITRL, Activation-inducible TNF-related ligand, GITRL, Glucocorticoid-induced TNF-related ligand, TL6, Tumor necrosis factor ligand superfamily member 18, hGITRL