This antibody is specific for CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1. Clone AKS1 is reported to activate lysis of FcgammaR-expressing cell line P815, by IL-2 activated NKp46+ cells.
纯化方法
Affinity Chromatography on Protein G.
免疫原
Fusion protein consisting of the extracellular region of bovine CD335. Spleen cells from immunised Balb/c mice were fused with cells of the NS-0 myeloma cell line.
Flow Cytometry: neat- 1/5, use 10 μL of the suggested working dilution to label 106 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 1 mL distilled water
浓度
0.1 mg/mL
缓冲液
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Lyophilized and reconstituted antibody ist stable at 2 - 8 °C for up to one month or at -20 °C for longer.
CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor. CD335 is primarily expressed by a subset natural killer (NK) cells. No expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.Synonyms: LY94, NCR1, NK cell-activating receptor, NKp46, Natural cytotoxicity triggering receptor, Natural killer cell p46-related protei