This antibody detects the 62 kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. This antibody may also be used to detect the commonly used c-myc tag.
交叉反应 (详细)
Species reactivity (tested):Human.
纯化方法
Affinity Chromatography on Protein G.
免疫原
Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of Human c-myc. Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line.
ELISA: 1/100-1/500. Western Blot: 1/100-1/500 (Non-Reducing Conditions). Detects a band of approximately 62 kDa in Colo 320HSR cell lysates. Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 1x10^6 cells in 100 μL. Membrane permeabilisation is required. Immunohistochemistry on Frozen and Paraffin Sections: This product does not requireprotein digestion pre-treatment of paraffin sections prior to staining. It does not requireantigen retrieval using heat treatment prior to staining of paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS containing 0.09 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
P62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.Synonyms: c-myc tag, myc tag, myc-tag