This antibody recognizes the 118 kDa cell surface glycoprotein CD146, also known as MUC18, Mel-CAM and S-endo. CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells. CD146 appears to act as an adhesion molecule. Expression in melanoma may be linked to tumor progression.
纯化方法
Affinity chromatography on Protein G
免疫原
Recombinant human MUC18 (D1-D5) Fc protein. Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.
Flow cytometry: 1/10 - 1/50, use 10 μL of the suggested working dilution to label 106 cells. Immunohistochemistry on frozen sections. Recommended positive control tissue: Melanoma. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells. CD146 appears to act as an adhesion molecule. Expression in melanoma may be linked to tumour progression.Synonyms: A32, CD146, Cell surface glycoprotein P1H12, MCAM, MUC18, Melanoma cell adhesion molecule, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen