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CD3 抗体 (Cytoplasmic Domain)

Name: CD3 抗体 (Cytoplasmic Domain)
SKU: ABIN119451
Availability: OutOfStock

CD3 适用: 人, 小鼠, 小鸡, Pig, 犬, 马 FACS, IHC (fro), IF, WB, IHC (p) 宿主: 大鼠 Monoclonal CD3-12 unconjugated

Staining of permeabilized, human peripheral blood lymphocytes with Rat anti Human CD3 (ABIN119451). (CD3 抗体 (Cytoplasmic Domain))

1 image

(3 references)

产品编号 ABIN119451

发货至: 中国

Datasheet as PDF

抗原 See all CD3 抗体

CD3
抗原表位
3

1

1

1

1
Cytoplasmic Domain
适用
176

97

24

19

16

10

8

7

7

7

5

2

2

1
人, 小鼠, 小鸡, Pig, 犬, 马
宿主
210

46

20

13

9

1
大鼠
Compatible Secondaries
克隆类型
288

6

1
单克隆
标记
92

39

32

23

23

11

8

6

5

5

5

4

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD3 antibody is un-conjugated
应用范围
273

81

57

52

45

37

31

22

9

7

7

6

4

3

2

2

2

2

2

2

2

2

1

1
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

序列

Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys

特异性

This antibody recognises a highly conserved epitope of the CD3 Molecule expressed by T lymphocytes. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species cross reactivity for the CD3 marker. (Jones et al. 1993, Kothlow et al. 2005).

交叉反应（详细）

Species reactivity (expected):Mammals, Birds.
Species reactivity (tested):Human, Mouse, Canine, Chicken, Equine, Porcine and Rhesus Monkey, Alpaca, Amazon Parrot, Duck, Harbour Porpoise, Koala, Raccoon, Sea Lion, Spotted Hyena.

纯化方法

Affinity Chromatography on Protein G

免疫原

Synthetic peptide sequence derived from Cytoplasmic epitope of CD3

克隆位点

CD3-12

亚型

IgG1

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应用备注

Flow cytometry (1/50-1/100, requires membrane permeabilisation). Immunohistochemistry on frozen sections and paraffin embedded tissues (1/1000). This product requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. 1 mM EDTA pH 8.0 is recommended for this purpose. Recommended positive control tissue: Tonsil. Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS, 0.09 % Sodium Azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store the reconstituted antibody undiluted at 2-8 °C.
Hebel, Eisinger, Neumeier, Rein-Fischboeck, Pohl, Meier, Boettcher, Froehner, Adams, Liebisch, Krautbauer, Buechler: "Lipid abnormalities in alpha/beta2-syntrophin null mice are independent from ABCA1." in: Biochimica et biophysica acta, Vol. 1851, Issue 5, pp. 527-36, (2015) (PubMed).

Hong, Puaux, Huang, Loumagne, Tow, Mackay, Kato, Prévost-Blondel, Avril, Nardin, Abastado: "Chemotherapy induces intratumoral expression of chemokines in cutaneous melanoma, favoring T-cell infiltration and tumor control." in: Cancer research, Vol. 71, Issue 22, pp. 6997-7009, (2011) (PubMed).

Shrestha, Hashiguchi, Ito, Miura, Takenouchi, Oyama, Kawahara, Tancharoen, Ki-I, Arimura, Yoshinaga, Noma, Shrestha, Nitanda, Kitajima, Arimura, Sato, Sakamoto, Maruyama: "B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4819-26, (2010) (PubMed).
抗原

CD3

别名

CD3 (CD3 产品)

背景

T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

基因ID

916

NCBI登录号

NP_000724

UniProt

P07766

途径

TCR Signaling, Ubiquitin Proteasome Pathway