This antibody recognises the CD8 cell surface antigen, a 30/32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells.
免疫原
Mouse L cells transfected with human CD8 gene. Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag.1.2.3 myeloma line.
Flow cytometry: use 10 μL of neat antibody to label 10^6 cells or 100 l whole blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.027 mg/mL
缓冲液
PBS, pH 7.2 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2