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His Tag 抗体

Name: His Tag 抗体
SKU: ABIN119353
Availability: OutOfStock

WB, IP, IHC (fro), EIA 宿主: 小鼠 Monoclonal AD1-1-10 unconjugated

Western blot of HIS-tagged protein probed with mouse anti histidine tag (ABIN119353) visualised with rabbit antimouse IgG : HRP (ABIN121365). (His Tag 抗体)

1 image

产品编号 ABIN119353

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag

适用

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宿主
92

60

11

9

5

4

1

1
小鼠
Compatible Secondaries
克隆类型
94

76

10
单克隆
标记
78

21

14

13

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
应用范围
141

92

38

27

25

19

15

14

14

13

13

7

7

5

4

4

4

3

3

3

2

2

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

序列

HHHHHH

纯化方法

Affinity Chromatography on Protein G

免疫原

PAX6 transcription factor linked to histidine tag. Remarks: Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

克隆位点

AD1-1-10

亚型

IgG1

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anti-His Tag antibody (HRP)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 HRP

anti-His Tag (C-Term) antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 Biotin

anti-His Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (DyLight 649)
WB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2 DyLight 649

应用备注

Immunhistology on Frozen Sections. ELISA. Immunoprecipitation. Western Blot (1/500-1/1000). In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS containing 0.09 % Sodium Azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原

His Tag

别名

6xHistidine Epitope Tag (His Tag 产品)

物质类

Tag

背景

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification.