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His Tag 抗体 (HRP)

Name: His Tag 抗体 (HRP)
SKU: ABIN119352
Availability: OutOfStock

WB, EIA 宿主: 小鼠 Monoclonal AD1-1-10 HRP

Western blot analysis of His-Ubiquitin recombinant protein probed with MOUSE ANTI HISTIDINE TAG:HRP (ABIN119352). (His Tag 抗体 (HRP))

1 image

产品编号 ABIN119352

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag

适用

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宿主
92

60

11

9

5

4

1

1
小鼠
Compatible Secondaries
克隆类型
94

76

10
单克隆
标记
79

20

14

13

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is conjugated to HRP
应用范围
142

100

39

27

25

19

15

14

14

13

13

11

7

5

5

4

4

3

3

3

2

2

1

1

1
Western Blotting (WB), Enzyme Immunoassay (EIA)

序列

HHHHHH

纯化方法

Affinity Chromatography on Protein G

免疫原

PAX6 transcription factor linked to histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

克隆位点

AD1-1-10

亚型

IgG1

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应用备注

ELISA: 1/20 - 1/1000. Western Blot: 1/1000 - 1/5000, In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS, pH 7.4 containing 0.01 % Thiomersal as preservative and a HRP stabilizer

储存条件

4 °C

储存方法

Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
抗原

His Tag

别名

6xHistidine Epitope Tag (His Tag 产品)

物质类

Tag

背景

6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.