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His Tag 抗体 (Biotin)

Name: His Tag 抗体 (Biotin)
SKU: ABIN119351
Availability: OutOfStock

WB, IP, IHC (fro), EIA 宿主: 小鼠 Monoclonal AD1-1-10 Biotin

Western blot of HIS tagged protein probed with mouse anti Histidine Tag - Biotin (ABIN119351) and visualised with Streptavidin - HRP (His Tag 抗体 (Biotin))

1 image

产品编号 ABIN119351

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag

适用

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宿主
92

60

11

9

5

4

1

1
小鼠
Compatible Secondaries
克隆类型
94

76

10
单克隆
标记
79

21

14

12

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is conjugated to Biotin
应用范围
141

92

38

27

25

19

15

14

14

13

13

7

7

5

4

4

4

3

3

3

2

2

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

序列

HHHHHH

纯化方法

Protein G affinity chromatography

免疫原

PAX6 transcription factor linked to histidine tag

克隆位点

AD1-1-10

亚型

IgG1

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WB, ELISA 宿主: 兔 Polyclonal unconjugated

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anti-His Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (DyLight 649)
WB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2 DyLight 649

应用备注

ELISA: 1/1000. Western Blot: 1/1000. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product is routinely tested in ELISA using histidine tagged ubiquitin and in Western blotting using histidine tagged recombinant protein control.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS with 0.09 % sodium azide as preservative

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原

His Tag

别名

6xHistidine Epitope Tag (His Tag 产品)

物质类

Tag