Huntingtin 抗体 (AA 1844-2131)
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- 抗原 See all Huntingtin (HTT) 抗体
- Huntingtin (HTT)
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抗原表位
- AA 1844-2131
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适用
- 人, 小鼠, 兔
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Huntingtin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody reacts with an epitope corresponding to the HDB region (amino acids 1844-2131) of the Huntingtin protein. The combined use of Clone HDB4E10 (Cat.-No SM1661), HDC8A4 (Cat.-No SM1662) and HDA3E10 (Cat.-No SM1660) demonstrate that huntingtin is enriched in neuronal cells in the brain.
- 纯化方法
- Purified
- 免疫原
- Recombinant protein corresponding to amino acids 1844 - 2131 of huntingtin
- 克隆位点
- HDB4E10
- 亚型
- IgG1
- Top Product
- Discover our top product HTT Primary Antibody
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- 应用备注
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Western Blot: Clone HDB4E10 detects a 350KD band on western blots but also detects smaller degradation products of huntingtin. Immunoprecipitation. Immunohistochemistry on frozen sections. Recommended positive control tissue: Brain.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, 0.09 % Sodium Azide
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Huntingtin (HTT)
- 别名
- Huntingtin (HTT 产品)
- 别名
- HTT antibody, hd antibody, ZHD antibody, CG9995 antibody, Dmel\\CG9995 antibody, HD antibody, Hsap\\HD antibody, Htt antibody, dHtt antibody, dhtt antibody, SLC6A4 antibody, huntington antibody, it15 antibody, htt antibody, IT15 antibody, AI256365 antibody, C430023I11Rik antibody, Hd antibody, Hdh antibody, huntingtin antibody, HTT antibody, htt antibody, LOC373520 antibody, Htt antibody
- 背景
- Huntington's disease (HD) is a neurodegenerative disorder caused by an expanding polyglutamine repeat in the huntingtin gene. HD is a mid-life onset autosomal dominant neurodegeneative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years. The HD gene product is widely expressed in human tissues, with the highest level of expression in the brain. The huntingtin gene product is expressed at similar levels in patients and controls, which suggests that the expansion of the polyglutamine repeat induces a toxic gain of function perhaps through interactions with other cellular proteins. Using yeast two-hybrid system, HAP1 (huntingtin associated protein 1) has been identified, that associates with huntingtin protein. The In vitro data suggest that the association between HAP1 and huntingtin is enhanced by increasing length of glutamine repeat.Synonyms: HD, HTT, Huntington Disease Protein, IT15
- 基因ID
- 3064
- NCBI登录号
- NP_002102
- UniProt
- P42858
- 途径
- PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport
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