2C11 recognises both native and denatured TSH receptor (binding to an epitope at the carboxy terminus between amino acids 354 and 359). It does inhibit binding of TSH. No cross reactivity has been observed with related LH and FSH receptors. This antibody recognises the mutant TSH receptor known as I167N as well as the wild type molecule.
纯化方法
Affinity chromatography on Protein G
免疫原
Recombinant Human TSH receptor. Spleen cells from immunised BALB/c mice were fused with cells of the NS1/Ag4.1 mouse myeloma cell line.
Flow Cytometry. ELISA. Immunoprecipitation: 5-10 μg/mL. Western Blot: 5-10 μg/mL. Functional Assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
The thyroid stimulating hormone (TSH) receptor (otherwise known as the thyrotropin receptor) is an important molecule in controlling the growth and function of the normal thyroid.Synonyms: LGR3, TSH-R, TSH-receptor, Thyroid-stimulating hormone receptor