The antibody reacts with Human Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Immunoprecipitation. Western Blotting (non-reducing conditions): 1-2 mg/mL, overnight in 4 °C. Positive control: HeLa human cervix carcinoma cell line MCF-7 human breast adenocarcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. SDS-PAGE (10 % separating gel). Immunohistochemistry (paraffin sections): 10 - 1:100. Positive tissue: epithelial tissue (skin). Immunocytochemistry. Positive control: MCF-7 human breast adenocarcinoma cell line HeLa human cervix carcinoma cell line. ELISA. Positive control: MCF-7 human breast adenocarcinoma cell line. Application note: The antibody DC-10 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the clone C-04. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH ~7.4, 15 mM Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the original vial at 2-8 °C. Stock solution and aliquots thereof should be stored at -20 °C.
Blumenthal, Giebel, Ummanni, Schlüter, Endlich, Endlich: "Morphology and migration of podocytes are affected by CD151 levels." in: American journal of physiology. Renal physiology, Vol. 302, Issue 10, pp. F1265-77, (2012) (PubMed).
Hachiya, Sriwiriyanont, Fujimura, Ohuchi, Kitahara, Takema, Kitzmiller, Visscher, Tsuboi, Boissy: "Mechanistic effects of long-term ultraviolet B irradiation induce epidermal and dermal changes in human skin xenografts." in: The American journal of pathology, Vol. 174, Issue 2, pp. 401-13, (2009) (PubMed).
Kirby, Beattie, Maroof, van Rooijen, Kaye: "SIGNR1-negative red pulp macrophages protect against acute streptococcal sepsis after Leishmania donovani-induced loss of marginal zone macrophages." in: The American journal of pathology, Vol. 175, Issue 3, pp. 1107-15, (2009) (PubMed).
Lau, Mollnau, Eiserich, Freeman, Daiber, Gehling, Brümmer, Rudolph, Münzel, Heitzer, Meinertz, Baldus: "Myeloperoxidase mediates neutrophil activation by association with CD11b/CD18 integrins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 2, pp. 431-6, (2005) (PubMed).
Vojt?sek, Stasková, Nenutil, Lauerová, Kovarík, Rejthar, Bártková, Bártek: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, (1990) (PubMed).
Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).
CK18 antibody, K18 antibody, Krt1-18 antibody, CYK18 antibody, DreK18 antibody, cb83 antibody, dapk1 antibody, sb:cb83 antibody, wu:fa13f02 antibody, wu:fb36b04 antibody, krt18 antibody, MGC64569 antibody, MGC75922 antibody, KRT18 antibody, CK-18-A antibody, K18-A antibody, NC-11 antibody, cyk18 antibody, k18 antibody, krt18-a antibody, krt18-b antibody, krt18a antibody, xkendob antibody, keratin 18 antibody, keratin 18, type I L homeolog antibody, keratin 18, type I antibody, keratin, type I cytoskeletal 18 antibody, K18, simple type I keratin antibody, Keratin, type I cytoskeletal 18 antibody, keratin 18, type I S homeolog antibody, Krt18 antibody, KRT18 antibody, krt18 antibody, krt18.L antibody, LOC700569 antibody, LOC100136778 antibody, k1c18 antibody, LOC100008885 antibody, krt18.S antibody
背景
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18