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Cytokeratin 18 抗体

Name: Cytokeratin 18 抗体
SKU: ABIN118968
Availability: OutOfStock

This anti-Cytokeratin 18 antibody is a 小鼠单克隆 antibody detecting Cytokeratin 18 in WB, IF, IHC (p), IP 和 EIA. Suitable for 人. This Primary Antibody has been cited in 6+ publications.

(6 references)

产品编号 ABIN118968

发货至: 中国

Quick Overview for Cytokeratin 18 抗体 (ABIN118968)

抗原

See all Cytokeratin 18 (KRT18) 抗体

Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This Cytokeratin 18 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

克隆位点

DC-10

Discover our top product KRT18 Primary Antibody

特异性

The antibody reacts with Human Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.

纯化方法

Affinity Chromatography on Protein A

免疫原

Human breast carcinoma cell line PMC-42

亚型

IgG1
anti-Keratin 18 (KRT18) (AA 53-150) antibody
KRT18 适用: 人, 小鼠, 大鼠, Pig WB, FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Keratin 18 (KRT18) antibody
KRT18 适用: 人 WB, ELISA, IHC (p), IP, ICC, ICFC 宿主: 小鼠 Monoclonal DC-10 unconjugated

anti-Keratin 18 (KRT18) antibody
KRT18 适用: 哺乳动物 WB, ELISA, IHC (p), IP, ICC, ICFC 宿主: 小鼠 Monoclonal C-04 unconjugated

anti-Keratin 18 (KRT18) (AA 238-396) antibody
KRT18 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Keratin 18 (KRT18) (AA 1-423) antibody
KRT18 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Keratin 18 (KRT18) (AA 238-396) antibody
KRT18 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal E4 unconjugated

anti-Keratin 18 (KRT18) (AA 72-380) antibody
KRT18 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Keratin 18 (KRT18) (AA 102-396) antibody
KRT18 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Keratin 18 (KRT18) antibody
KRT18 适用: 人 WB, IHC, IF, FACS, StM, ISt 宿主: 兔 Monoclonal KRT18-2808R unconjugated Recombinant Antibody

anti-Keratin 18 (KRT18) antibody
KRT18 适用: 人, 犬, Cat WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal DE-K18 unconjugated

应用备注

Immunoprecipitation. Western Blotting (non-reducing conditions): 1-2 mg/mL, overnight in 4 °C. Positive control: HeLa human cervix carcinoma cell line MCF-7 human breast adenocarcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. SDS-PAGE (10 % separating gel). Immunohistochemistry (paraffin sections): 10 - 1:100. Positive tissue: epithelial tissue (skin). Immunocytochemistry. Positive control: MCF-7 human breast adenocarcinoma cell line HeLa human cervix carcinoma cell line. ELISA. Positive control: MCF-7 human breast adenocarcinoma cell line. Application note: The antibody DC-10 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the clone C-04.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS, pH ~7.4, 15 mM Sodium Azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store the original vial at 2-8 °C. Stock solution and aliquots thereof should be stored at -20 °C.
Blumenthal, Giebel, Ummanni, Schlüter, Endlich, Endlich: "Morphology and migration of podocytes are affected by CD151 levels." in: American journal of physiology. Renal physiology, Vol. 302, Issue 10, pp. F1265-77, (2012) (PubMed).

Hachiya, Sriwiriyanont, Fujimura, Ohuchi, Kitahara, Takema, Kitzmiller, Visscher, Tsuboi, Boissy: "Mechanistic effects of long-term ultraviolet B irradiation induce epidermal and dermal changes in human skin xenografts." in: The American journal of pathology, Vol. 174, Issue 2, pp. 401-13, (2009) (PubMed).

Kirby, Beattie, Maroof, van Rooijen, Kaye: "SIGNR1-negative red pulp macrophages protect against acute streptococcal sepsis after Leishmania donovani-induced loss of marginal zone macrophages." in: The American journal of pathology, Vol. 175, Issue 3, pp. 1107-15, (2009) (PubMed).

Lau, Mollnau, Eiserich, Freeman, Daiber, Gehling, Brümmer, Rudolph, Münzel, Heitzer, Meinertz, Baldus: "Myeloperoxidase mediates neutrophil activation by association with CD11b/CD18 integrins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 2, pp. 431-6, (2005) (PubMed).

Vojt?sek, Stasková, Nenutil, Lauerová, Kovarík, Rejthar, Bártková, Bártek: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, (1990) (PubMed).

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).
抗原

Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

别名

Cytokeratin 18

背景

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18

基因ID

3875

NCBI登录号

NP_000215

UniProt

P05783

途径

Apoptosis, Caspase Cascade in Apoptosis