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F-Actin 抗体

适用: 人, 小鼠, 大鼠, 兔 FACS, WB, IHC (fro), EIA 宿主: 小鼠 Monoclonal NH3 unconjugated
产品编号 ABIN118960
发货至: 中国
  • 抗原 See all F-Actin products
    F-Actin
    适用
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    人, 小鼠, 大鼠, 兔
    宿主
    • 2
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    小鼠
    克隆类型
    • 2
    • 1
    单克隆
    标记
    • 3
    This F-Actin antibody is un-conjugated
    应用范围
    • 3
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    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    特异性
    This antibody recognizes Human Filamentous Actin (F-actin). The antibody binds to the N-terminal region of Actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. Clone NH3 is reported to recognise Actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule. Clone NH3 is also described to show reactivity with a 43 kDa polypeptide using cell lines U937 and HL-60 by SDS/PAGE and Immunoblotting.
    纯化方法
    Purified
    免疫原
    Human monocytes and U937 cell line. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
    克隆位点
    NH3
    亚型
    IgM
  • 应用备注
    Flow Cytometry (1:10). Immunohistochemistry on frozen and paraffin sections. This product may require protein digestion pretreatment (e.g. trypsin or pronase) or microwave antigen retrieval prior to staining of paraffin sections. ELISA (1:10) Western blotting (1:100 - 1:500)
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS, 0.09 % Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原
    F-Actin
    别名
    Actin (F-Actin) (F-Actin 产品)
    背景
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. G-Actin (globular actin) with bound ATP can polymerise to form F-Actin (Filamentious Actin). Actin monomers spiral ariound the axis of the filament similar to a double helix. F-Actin may also undergo a process called treadmilling, in which filament length remains constant and actin monomers add at one end and dissociated at the other. Synonyms: Actin F type
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