This antiobdy recognises Mast Cell Tryptase, both alpha and beta isoforms. This antibody is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology.
纯化方法
Affinity Chromatography on Protein G
免疫原
Human mast cell tryptase purified from Human lung tissue. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
ELISA. Western Blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/10,000): This product requires either protein digestion pre-treatment of paraffin sections e.g. pronase, or HIER, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4, 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation.Synonyms: Mast Cell Tryptase, TPSB1, Tryptase I