This antibody recognises the C5a receptor (C5aR) CD88. Clone S5/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognise the heptameric peptide (D15DKDTLD21). Clone S5/1 has been shown to inhibit the binding of C5a to its receptor. We recommend the use of SM1182A for functional studies.
交叉反应 (详细)
Species reactivity (expected):Bovine and Rabbit. Species reactivity (tested):Human
纯化方法
Ammonium sulphate precipitation
免疫原
C5aR - peptide: Met1 - Asn31. Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.
Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 5 x 10e5 cells in 100 μL. Immunohistochemistry on paraffin sections. Western Blot. Functional Assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS containing 0.09 % Sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.