This antibody reacts with the heavy chain of the CD98 Molecule. This antibody binds to all cell lines in culture and to activated Tcells. Note: The epitope is distinct from clone 44H7 (SM1173), but the same as recognised by 4F2.
交叉反应 (详细)
Species reactivity (tested):Human.
纯化方法
Affinity Chromatography on Protein G.
免疫原
HOON pre-B leukaemia cell line. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
Flow Cytometry: Use 10 μL of 1/50-1/200 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
SLC3A2
(Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))
4F2 antibody, 4F2HC antibody, 4T2HC antibody, CD98 antibody, CD98HC antibody, MDU1 antibody, NACAE antibody, Mdu1 antibody, MGC53951 antibody, slc3a2 antibody, MGC53965 antibody, SLC3A2 antibody, AI314110 antibody, Cd98 antibody, Ly-10 antibody, Ly-m10 antibody, Ly10 antibody, Mgp-2hc antibody, fc65h12 antibody, wu:fc65h12 antibody, id:ibd1327 antibody, sb:cb519 antibody, wu:fe14a07 antibody, wu:fq18d12 antibody, zgc:55813 antibody, solute carrier family 3 member 2 antibody, solute carrier family 3 (amino acid transporter heavy chain), member 2 L homeolog antibody, solute carrier family 3 (amino acid transporter heavy chain), member 2 S homeolog antibody, solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 antibody, solute carrier family 3 (amino acid transporter heavy chain), member 2a antibody, solute carrier family 3 (amino acid transporter heavy chain), member 2b antibody, SLC3A2 antibody, Slc3a2 antibody, slc3a2.L antibody, slc3a2.S antibody, slc3a2a antibody, slc3a2b antibody
背景
CD98 is a type II transmembrane glycoprotein which associates with members of the L-type amino transporter family to form a heterodimeric complexCD98 constitutes the heavy chain of the complex and is required for the surface expression and basolateral localization of the amino acid transporter complex in polarized epithelial cells. The CD98 heavy chain also interacts with beta 1 integrins and regulates their activation through the cytoplasmic domains and transmembrane regions. Studies suggest that overexpression of CD98 may contribute to cell growth and survival by regulating integrin signalling, and therefore may play an important role in tumorigenesis. The tissue distribution of this antigen is broad. Monocytes express high levels of CD98 antigen. Peripheral blood T and B cells, as well as NK cells and granulocytes express low levels of CD98 antigen. Activation of T cells and NK cells leads to upregulation of this antigen.Synonyms: 4F2 cell-surface antigen heavy chain, 4F2 heavy chain antigen, 4F2hc, Lymphocyte activation antigen 4F2 large subunit, MDU1