This antibody reacts with CD79a positive cells by flow cytometry and ELISA specific for a fusion protein of CD79a-Fc. This clone has been reported to be useful in distinguishing B-CLL from mantle cell lymphoma in flow cytometric assays. (2) The antibody has been reported to induce phosphorylation of syk kinase. (3)
纯化方法
Affinity Chromatography on Protein A.
免疫原
IgM complex isolated from Daudi cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Flow Cytometry: Use 10 μL of 10 μg/mL diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation: 20 μg/mL. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
CD79a (CD79A)
(B-cell antigen receptor complex-associated protein alpha chain (CD79A))
A disulfide-linked heterodimer, consisting of CD79a / mb1 and CD79b / B29 polypeptides, is non-covalently associated with membrane bound immunoglobulins on B cells to constitute the B cell Ag receptor. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling.Synonyms: B-Cell marker, B-cell antigen receptor complex-associated protein alpha-chain, IGA, MB-1 membrane glycoprotein, MB1, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein