This antibody recognizes CD62P. It reacts with a 140 kDa antigen expressed on activated platelets and also on endothelial cells both of blood and lymph vessels.
Flow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections (1/1000-1/5000) and Paraffin Embedded Sections (1/500-1/2000): This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but staining may be enhanced using Sodium Citrate buffer pH 6.0. Recommended Positive Control Tissue: Tonsil. Western blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4, 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing. Dilute only prior to immediate use.
储存条件
4 °C/-20 °C
储存方法
Store the product undiluted at 2-8 °C for up to one month. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.
抗原
P-Selectin (SELP)
(Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
CD62P (P-Selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.Synonyms: CD62 antigen-like family member P, GMP-140, GMRP, GRMP, Granule membrane protein 140, LECAM3, Leukocyte-endothelial cell adhesion molecule 3, PADGEM, SELP