This antibody is specific for Mouse Interleukin-4 (IL-4). Clone BVD4-1D11 is reported to neutralise the effects of IL-4. We recommend the use of SM101A for functional studies.
无交叉反应
大鼠
交叉反应 (详细)
Species reactivity (tested):Mouse.
纯化方法
Affinity Chromatography on protein G
免疫原
Recombinant Mouse IL-4. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required. Immunohistochemistry on frozen sections. ELISA: 1 μg/mL - 5 μg/mL. Western Blot: 1 μg/mL - 5 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS containing 0.09 % Sodium Azide as preservative
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Interleukin-4 is a potent lymphoid growth factor, which stimulates the growth and survival of certain B and T cells.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra